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For years, the residents of a small Mexican town complained about large numbers of flies coming from a nearby hog farm, where millions of pigs are raised each year. In 2009, the majority of residents in this town became sick with "swine flu," caused by the H1N1 influenza virus. This virus infects people but is apparently genetically similar to a virus that infects hogs. The virus has since gone on to infect (and, in some cases, kill) people in Mexico City, has quickly spread around the entire planet, and has been declared a global pandemic. Based on this scenario and your knowledge of genetics, this virus is most likely:


A) inappropriately called the "swine flu" virus and is merely coincidentally similar to a virus that infects pigs.
B) a genetically unmodified virus that first infected pigs, then flies, then people.
C) a human virus that was transformed within the bacteria living in pig manure and can now infect pigs and humans.
D) the result of a hog-specific virus being transformed into a human-specific virus, after being carried from the hog farm to people via flying insects.
E) the first known example of a genetically modified virus that could potentially cause millions of people to die or perhaps the extinction of humans altogether.

F) B) and E)
G) C) and D)

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All of the following are goals of biotechnology EXCEPT:


A) generating economic benefits.
B) efficiently producing biologically important molecules.
C) improving agriculturally important food plants.
D) treating disease more effectively.
E) creating humans with higher intelligence levels.

F) A) and B)
G) A) and C)

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Recombinant DNA technology:


A) will never be of economic importance.
B) involves only changing genes in large mammals.
C) randomly creates new genes from free nucleotides.
D) is always dangerous and inevitably leads to mutations and mistakes.
E) involves combining existing genes from different organisms.

F) None of the above
G) B) and D)

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The DNA of humans and chimps differs by just over 1%. True or False?

A) True
B) False

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Which of the following is NOT an example of genetic recombination between different species in nature?


A) bacterial transformation
B) bacteria that acquire foreign plasmids
C) viruses that transfer DNA between host organisms
D) sexual reproduction

E) A) and B)
F) B) and D)

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Which of the following molecular techniques involves "DNA replication in a tube"?


A) transformation
B) gel electrophoresis
C) PCR
D) RFLP

E) All of the above
F) C) and D)

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Which pair of enzymes is necessary to make recombinant DNA?


A) DNA polymerase and ligase
B) ligase and restriction enzyme
C) restriction enzyme and DNA polymerase
D) DNA polymerase and RNA polymerase

E) A) and D)
F) C) and D)

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In gel electrophoresis, DNA is separated based on charge and ________.

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Restriction enzymes:


A) randomly cut DNA into small pieces.
B) produce single-stranded DNA fragments with "sticky ends."
C) can cut only bacterial DNA.
D) will cut DNA only if it contains methyl groups.

E) C) and D)
F) None of the above

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How many copies of a gene of interest are produced from one template DNA strand after three cycles of the polymerase chain reaction (PCR) ?


A) two
B) three
C) four
D) five
E) eight

F) A) and E)
G) B) and D)

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All of the following are benefits of sequencing the human genome EXCEPT:


A) genes were discovered whose functions were previously unknown.
B) more genes that cause disease have been discovered.
C) the number of genetic disorders that can be diagnosed with molecular techniques has increased.
D) better comparisons can be made between human genetics and the genetics of other organisms.
E) every genetic disorder is now curable or preventable.

F) D) and E)
G) A) and E)

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An organism that contains altered genes is considered all of the following EXCEPT:


A) transgenic.
B) a genetically modified organism (GMO) .
C) genetically engineered.
D) polymorphic.

E) A) and D)
F) B) and D)

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How is it possible for a dead bacterial cell to influence the DNA in a living bacterial cell?

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Dead bacteria release plasmids...

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The earliest examples of biotechnology included:


A) the use of genetic markers to learn more about genetic inheritance.
B) the study and treatment of genetic diseases in people.
C) the careful examination of patterns of bacterial inheritance.
D) the use of selective breeding to provide better, more nutritious food.

E) C) and D)
F) B) and D)

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Plasmids are:


A) noncircular DNA segments found only in bacteria.
B) small, replicating rings of DNA found in bacteria.
C) chromosomal loops made of RNA.
D) found only in three species of bacteria.
E) necessary in order for bacteria to reproduce.

F) B) and D)
G) C) and D)

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Provide one example of an agricultural application of biotechnology.

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Possible answers are selective...

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The history of biotechnology spans:


A) fewer than 50 years.
B) around 100 to 150 years.
C) about 1,000 years.
D) at least 10,000 years.

E) All of the above
F) A) and C)

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The polymerase chain reaction joins many small DNA molecules into a long chain. True or False?

A) True
B) False

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A radioactively labeled DNA ________ can be used to identify a specific piece of DNA in an electrophoresis gel.

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To make insect-resistant, transgenic plants, ________ are first inserted into ________.


A) Bt genes; Agrobacterium tumefaciens bacteria
B) Bt genes; disabled Ti plasmids
C) disabled Ti plasmids; A. tumefaciens bacteria
D) disabled Ti plasmids; Bt genes
E) A) tumefaciens bacteria; target plant cells

F) D) and E)
G) A) and B)

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