Question 1

Shown below is a diagram for a plasmid vector you want to use to clone a gene.The diagram shows the location of the recognition sites for four restrictions enzymes,BamHI (B) ,EcoRI (E) ,HindIII (H) ,and XhoI (X) .The genes encoding beta-lactamase (AmpR) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning? R) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning?   A) BamHI B) EcoRI C) HindIII D) XhoI E) BamHI or XhoI would be equally useful. " class="answers-bank-image d-inline" rel="preload" >

Accepted Answer

A)  BamHI
B)  EcoRI
C)  HindIII
D)  XhoI
E)  BamHI or XhoI would be equally useful.F) B) and C)
G) A) and E)

Question 2

What is TRUE of restriction enzymes?

Accepted Answer

A)  they randomly cleave DNA at sites within a genome
B)  they catalyze the formation of hydrogen bonds between DNA fragments
C)  they protect bacterial cells from invasion by foreign DNA
D)  they are not naturally produced by bacteria,but are bioengineered by humans
E)  there are less than 10 restriction enzymes knownF) C) and E)
G) B) and C)

Question 3

You are working in a cancer lab and are interested in identifying some genes that are important in the onset of lung cancer.You have several genes you think may be involved,so you "print" spots of the DNA sequences of interest on a microarray slide.After doing this,you isolate mRNA from both normal lung cells and cancerous lung cells.Before you use these mRNAs to probe the microarray slide,what do you need to do?

Accepted Answer

A)  make cDNA and amplify the cDNA using PCR
B)  sequence the mRNA
C)  clone each mRNA into a BAC vector
D)  amplify the mRNA using PCR
E)  add reverse transcriptase and fluorescent nucleotides to the mRNA to make cDNAF) None of the above
G) All of the above

Question 4

Restriction enzymes are invaluable tools in gene cloning because

Accepted Answer

A)  they cut both strands of DNA at specific sites
B)  they replicate the gene of interest
C)  they can produce sticky ends so DNA from different sources can be joined together
D)  they cut at specific sites within the DNA and produce sticky ends allowing DNA from different sources to be joined together
E)  they replicate the DNA of interest,they cut at specific sites within the DNA,and produce sticky ends allowing DNA from different sources to be joined togetherF) C) and E)
G) All of the above

Question 5

The protein p53 is a tumor suppressor - it regulates the cell cycle and is important in preventing cancer in humans.What phenotype would you expect in p53 knockout mice?

Accepted Answer

A)  cells of p53 knockout mice would not be able to go through mitosis
B)  cells of p53 knockout mice would not be able to go through meiosis
C)  p53 knockout mice would be less susceptible to cancer
D)  p53 knockout mice would be more susceptible to cancersE) B) and C)
F) A) and D)

Question 6

Which of the following stretches of double-stranded DNA is mostly likely to be recognized as a restriction enzyme cutting site and create fragments with sticky ends?

Accepted Answer

A)  5'-GTTCAT-3'3'-CAAGTA-5'
B)  5'-ACCCCT-3'3'-TGGGGA-5'
C)  5'-TCCGGA-3'3'-AGGCCT-5'
D)  5'-TTTTTT-3'3'-AAAAAA-5'
E)  5'-GAATCG-3'F) All of the above
G) A) and C)

Question 7

A plasmid that has genes for both tetracycline resistance and ampicillin resistance is cut with the restriction enzyme BamHI.BamHI cuts within the ampicillin resistance gene.Caenorhabditis elegans DNA that has been cut with BamHI is ligated into the linearized plasmid to make a genomic DNA library.The mixture is transformed into

Accepted Answer

A)  Both ampicillin and tetracycline are needed to make this determination.
B)  tetracycline
C)  Neither ampicillin or tetracycline are needed to make this determination.
D)  ampicillin
E)  coli.What antibiotic(s) ,if any,should be added to the medium to determine which cells contain plasmids?F) All of the above
G) A) and E)

Question 8

Most plasmids used in biotechnology contain a selectable marker such as an antibiotic-resistance (ampR)gene.

Accepted Answer

A) True 
 B)False

Question 9

You grow a culture of bacteria and split it into two culture tubes.One sample of bacteria you treat with calcium and the other is not treated.You then attempt to transform the two samples with a plasmid.Both samples survive,but only the samples treated with calcium take up the plasmid.What is the most likely explanation for your results?

Accepted Answer

A)  The bacteria use calcium as a source of energy to take up the DNA.
B)  DNA is degraded in the absence of calcium.
C)  The calcium makes the bacterial membrane more porous to DNA.
D)  Calcium precipitates DNA,preventing it from being taken up by cells.
E)  The bacteria require calcium to grow.F) A) and E)
G) C) and E)

Question 10

Shown below is the output from an automated sequencing reaction.What is the sequence of the DNA template strand?

Accepted Answer

A)  5' - CCTGATAGAGTCTCCCT - 3'.
B)  3' - CCTGATAGAGTCTCCCT - 5'
C)  3' - GGACTATCTCAGAGGGA - 5'
D)  5' - GGACTATCTCAGAGGGA - 3'
E)  5' - CCTGATAGAGTCTCCCT - 3'F) B) and E)
G) B) and C)

Question 11

A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme.The digested plasmid and chromosomal DNA were incubated together with DNA ligase and transformed into bacteria.Plasmids isolated from some of the colonies do not contain inserted chromosomal DNA.What is the most likely explanation for this observation?

Accepted Answer

A)  The chromosomal DNA did not have any restriction sites for the enzyme used.
B)  The two sticky ends of the plasmid can hybridize to both sticky ends of the chromosomal DNA fragments.
C)  The two sticky ends of the plasmid can hybridize back together,recircularizing.
D)  The two sticky ends of the vector can remain free or unhybridizeD.
E)  The two sticky ends of the plasmid can hybridize with only one end of the chromosomal DNA fragmentsF) D) and E)
G) All of the above

Question 12

Following treatment with restriction enzymes,what procedure would be used to separate DNA fragments of different lengths?

Accepted Answer

A)  transformation
B)  amplification
C)  gel electrophoresis
D)  polymerase chain reaction
E)  microarray analysisF) A) and C)
G) B) and E)

Question 13

Restriction enzymes bind to specific sequences of DNA to seal them together.

Accepted Answer

A) True 
 B)False

Question 14

Which of the following vectors must undergo transformation in order to achieve gene cloning?

Accepted Answer

A)  fungus
B)  bacteriophage
C)  yeast
D)  virus
E)  plasmidF) A) and E)
G) B) and D)

Question 15

Why is Taq polymerase used in a polymerase chain reaction (PCR) ?

Accepted Answer

A)  Taq polymerase has proofreading activity and thus makes fewer errors than other DNA polymerases.
B)  Taq polymerase is more efficient than other DNA polymerases.
C)  Taq polymerase can produce DNA from an RNA template,unlike other DNA polymerases.
D)  Unlike other DNA polymerases,Taq polymerase is not inhibited by dideoxy nucleotides.
E)  Unlike other DNA polymerases,Taq polymerase is heat stable and survives the 94 degree denaturation step in PCR.F) A) and E)
G) A) and B)

Question 16

What statement about the modified Ti plasmid used to make transgenic plants is accurate?

Accepted Answer

A)  it is derived from a virus that infects the bacterium Agrobacterium tumefaciens
B)  it causes crown gall formation
C)  it contains a screenable marker gene
D)  it contains a cDNA region that integrates into the plant cell's genome
E)  it has its restriction enzyme recognition sites removedF) B) and E)
G) All of the above

Question 17

To study the promoter of a gene,a researcher should use a cDNA library.

Accepted Answer

A) True 
 B)False

Question 18

The FtsZ gene in bacteria encodes an ancestral tubulin that is important in binary fission (the mechanism of cell division in prokaryotes) .To study the FtsZ gene in eukaryotes,researchers made an FtsZ knockout in the moss What statement about this knockout plant is accurate?

Accepted Answer

A)  The knockout plant is heterozygous for an abnormal eukaryotic FtsZ gene
B)  The knockout plant does not produce any normal FtsZ protein
C)  The knockout plant contains a copy of the normal bacterial FtsZ gene
D)  The knockout plant makes abnormal bacterial FtsZ protein
E)  The knockout plant makes half the normal amount of FtsZ proteinF) A) and E)
G) All of the above

Question 19

You cut a plasmid with a restriction enzyme that cuts it into three pieces - one fragment is 240 base pairs,one fragment is 600 base pairs,and one fragment is 1200 base pairs.You carry out electrophoresis of the digested sample.After ten minutes,which of the fragments,if any,will be closest to the top of the gel where the samples were inserted?

Accepted Answer

A)  the 240 base pair fragment
B)  the 600 base pair fragment
C)  the 1200 base pair fragment
D)  all three fragments will be equally near the top because of their identical sticky ends
E)  it depends on the charge of the DNA fragmentF) B) and D)
G) C) and E)

Question 20

Why is the production of transgenic plants somewhat easier than the production of transgenic animals?

Accepted Answer

A)  plant cells lack cell walls,so uptake of DNA is easier than in animal cells
B)  plant cells are more stable than animal cells
C)  plant cells are totipotent
D)  plant cells are more highly evolved as they contain both chloroplasts and mitochondria
E)  all of the above are reasons why plant cells are more easily transformed than animal cellsF) B) and C)
G) C) and E)

Exam 16: Genetic Technology

Which of the following vectors must undergo transformation in order to achieve gene cloning?

Correct Answer
verified

Correct Answer
verified

Most plasmids used in biotechnology contain a selectable marker such as an antibiotic-resistance (amp^R)gene.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Why is the production of transgenic plants somewhat easier than the production of transgenic animals?

Correct Answer
verified

Following treatment with restriction enzymes,what procedure would be used to separate DNA fragments of different lengths?

Correct Answer
verified

What is TRUE of restriction enzymes?

Correct Answer
verified

Shown below is the output from an automated sequencing reaction.What is the sequence of the DNA template strand?

Correct Answer
verified

The FtsZ gene in bacteria encodes an ancestral tubulin that is important in binary fission (the mechanism of cell division in prokaryotes) .To study the FtsZ gene in eukaryotes,researchers made an FtsZ knockout in the moss What statement about this knockout plant is accurate?

Correct Answer
verified

The protein p53 is a tumor suppressor - it regulates the cell cycle and is important in preventing cancer in humans.What phenotype would you expect in p53 knockout mice?

Correct Answer
verified

Restriction enzymes are invaluable tools in gene cloning because

Correct Answer
verified

Why is Taq polymerase used in a polymerase chain reaction (PCR) ?

Correct Answer
verified

Restriction enzymes bind to specific sequences of DNA to seal them together.

Correct Answer
verified

Which of the following stretches of double-stranded DNA is mostly likely to be recognized as a restriction enzyme cutting site and create fragments with sticky ends?

Correct Answer
verified

Correct Answer
verified

What statement about the modified Ti plasmid used to make transgenic plants is accurate?

Correct Answer
verified

Correct Answer
verified

To study the promoter of a gene,a researcher should use a cDNA library.

Correct Answer
verified

Exam 16: Genetic Technology

Which of the following vectors must undergo transformation in order to achieve gene cloning?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Most plasmids used in biotechnology contain a selectable marker such as an antibiotic-resistance (ampR)gene.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Why is the production of transgenic plants somewhat easier than the production of transgenic animals?

Correct AnswerverifiedShow Answer

Following treatment with restriction enzymes,what procedure would be used to separate DNA fragments of different lengths?

Correct AnswerverifiedShow Answer

What is TRUE of restriction enzymes?

Correct AnswerverifiedShow Answer

Shown below is the output from an automated sequencing reaction.What is the sequence of the DNA template strand?

Correct AnswerverifiedShow Answer

The FtsZ gene in bacteria encodes an ancestral tubulin that is important in binary fission (the mechanism of cell division in prokaryotes) .To study the FtsZ gene in eukaryotes,researchers made an FtsZ knockout in the moss What statement about this knockout plant is accurate?

Correct AnswerverifiedShow Answer

The protein p53 is a tumor suppressor - it regulates the cell cycle and is important in preventing cancer in humans.What phenotype would you expect in p53 knockout mice?

Correct AnswerverifiedShow Answer

Restriction enzymes are invaluable tools in gene cloning because

Correct AnswerverifiedShow Answer

Why is Taq polymerase used in a polymerase chain reaction (PCR) ?

Correct AnswerverifiedShow Answer

Restriction enzymes bind to specific sequences of DNA to seal them together.

Correct AnswerverifiedShow Answer

Which of the following stretches of double-stranded DNA is mostly likely to be recognized as a restriction enzyme cutting site and create fragments with sticky ends?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

What statement about the modified Ti plasmid used to make transgenic plants is accurate?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

To study the promoter of a gene,a researcher should use a cDNA library.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Most plasmids used in biotechnology contain a selectable marker such as an antibiotic-resistance (amp^R)gene.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified