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Differentiate between structural, functional, and comparative genomics.

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Genomics is the study of genes and their...

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The function of DNA ligase in recombinant technology is to


A) cut DNA into many fragments.
B) carry DNA into a new cell.
C) seal DNA into an opening created by restriction enzymes.
D) make millions of copies of a specific segment of DNA.
E) separate fragments of DNA by their length and electrical charges.

F) A) and B)
G) A) and E)

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The function of the polymerase chain reaction in genetic engineering is to


A) cut DNA into many fragments.
B) carry DNA into a new cell.
C) link together newly joined fragments of DNA.
D) make multiple copies of a specific segment of DNA.
E) separate fragments of DNA by their length and electrical charges.

F) B) and E)
G) B) and D)

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In what field of study would scientists predict a protein's three-dimensional shape and how DNA mutations would affect protein function.


A) bioinformatics
B) proteomics
C) genomics
D) All of the answer choices.

E) B) and D)
F) B) and C)

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When using bacteria to clone a human gene and express its product, as in the case of insulin, the gene cannot contain introns because bacteria do not have the enzymes to process mRNA. How do scientists overcome this challenge?

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Reverse transcriptase is used to "revers...

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Gene cloning can be accomplished by recombinant DNA technology and polymerase chain reactions.

A) True
B) False

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The term "ligase" adds the suffix "-ase" to the Latin root word for


A) gene.
B) work.
C) cut.
D) bind.
E) copy-producing.

F) B) and E)
G) None of the above

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The introduction of normal genes into an afflicted individual for therapeutic use is called


A) human cloning.
B) proteonomics.
C) gene therapy.
D) genetic profiling.

E) C) and D)
F) A) and C)

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Which of the following is mismatched?


A) bioinformatics - the study of a genomic and proteomic information using computer analysis
B) polymerase chain reaction - process that separates DNA fragments according to size
C) genomics - the study of the genomes of humans and other organisms
D) proteomics - the study of species' proteins

E) B) and D)
F) A) and D)

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Place the following steps in cloning DNA in the correct order 1 - use vector to deliver new rDNA to bacterial or other cells 2 - use restriction enzymes to cut a plasmid and add the desired gene 3 - isolate and cut out a desired gene using restriction enzymes 4 - use DNA ligase to seal the new gene 5 - allow bacterial cells to replicate and produce desired product


A) 1, 2, 3, 4, 5
B) 5, 3, 2, 4, 1
C) 2, 5, 3, 1, 4
D) 3, 2, 4, 1, 5

E) A) and B)
F) All of the above

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What is the evolutionary advantage of having multiple copies of a gene?

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Scientists hypothesize that it may be ad...

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Automated polymerase chain reactions were made possible when the temperature insensitive DNA polymerase was isolated from the thermophile Thermus aquaticus.

A) True
B) False

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DNA fingerprinting may be used to establish paternity. Analyze the banding pattern pictured at right to determine the father of the child. How did you arrive at your answer? DNA fingerprinting may be used to establish paternity. Analyze the banding pattern pictured at right to determine the father of the child. How did you arrive at your answer?

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Male 1 is the father of the child. The c...

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Animal cloning is a difficult process with a low success rate.

A) True
B) False

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Describe the procedure used to produce a transgenic cow that will produce human growth hormone in their milk.

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Answers may vary but should include:
1. ...

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Gene therapy is being used in cancer patients to make healthy cells more tolerant of chemotherapy and to make tumors more vulnerable to chemotherapy.

A) True
B) False

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It is estimated that humans have approximately ______ base pairs in our genome.


A) 3 billion
B) 2.5 million
C) 180 million
D) 12 trillion

E) A) and C)
F) A) and B)

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A common method used to introduce rDNA into bacterial host cells is


A) viral infection.
B) bacteriophage infection.
C) plasmid transfer.
D) microinjection of DNA.
E) laser irradiation of cells in a fluid containing DNA.

F) B) and D)
G) A) and D)

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Familial hypercholesterolemia


A) is characterized by high blood levels of cholesterol.
B) is a condition that develops when liver cells lack a receptor protein for removing cholesterol from the blood.
C) may be treated by ex vivo gene therapy.
D) All of the answer choices are true.
E) Only two of the answer choices are true.

F) D) and E)
G) A) and B)

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What is the function of gel electrophoresis in genetic engineering?


A) cut DNA into many fragments
B) carry DNA into a new cell
C) link together newly joined fragments of DNA
D) make millions of copies of a specific segment of DNA
E) separate fragments of DNA by their length and electrical charges

F) A) and E)
G) A) and B)

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